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 Wako Autokit Micro Albumin Kit, Calibrators and Controls The Wako Autokit Micro Albumin assay is liquid, ready to use and sensitive utilizing an in vitro turbidimetric methodology to detect Micro Albumin in human urine and can be automated using your existing clinical chemistry instrumentation. Wako's Autokit Micro Albumin employs the use of an anti-human albumin antibody which combines specifically with albumin in the sample. This mixture yields an insoluble aggregate. The amount of albumin is proportional to the turbidity in the sample and is measured optically at 700nm. Wako's Autokit Micro Albumin assay has excellent performane. The within run precision is 1.81%CV and 0.75%CV for mean concentrations of 4.97 micro g/mL and 105.81 micro g/mL respectively. Linearity of the assay extends up to 500 micro g/mL and the prozone phenomenon is not observed up to 10,000 micro g/mL. Micro Albumin Calibrator set and two level control set are also now available from Wako. The Micro Albumin Calibrator set contains 5 levels of calibrators and are stored at 2-10 degrees C. A two level control set is available with target ranges of 40 +/- 8 micro g/mL and 100 +/- 20 micro g/mL for level 1 and level 2 respectively. For more information about Wako's Autokit Micro Albumin assay or a list of available insturment applications, please contact Wako Diagnostics, 1600 Bellwood Road, Richmond, VA 23237, Toll free: 877-714-1924, Direct: 804-714-1924, Facsimile: 804- 271-0449, or by E-mail: Diagnostics@wakousa.com. Wako Prealbumin Test System Wako Diagnostics, a division of Wako Chemicals USA, is pleased to announce the availability of its Prealbumin Test System for the quantitative determination of prealbumin in serum or plasma. Prealbumin, also called transtheritin, is a serum protein with molecular with molecular weight of approximately 55,000. The concentration of prealbumin in blood has proven to be useful in assessment of nutritional status and is commonly measures in hospitalized patients. The Wako Prealbumin assay utilizes turbidimetric immunoassay method for measuring the concentration of prealbumin in serum or plasma. When a sample is mixed with the assay reagents, the prealbumin in the sample binds specifically with anti-human prealbumin antibody to yield an insoluble aggregate resulting in increased turbidity in the reaction mixture. The degree of the resulting turbidity is proportional to the prealbumin concentration in the sample and can be measured photometrically. The Wako prealbumin test has excellent precision and accuracy as well as specificity. The linear range of the assay is up to 60 mg/dL. The method is easily adaptable to most automated clinical analyzers. For more information about Wako's Prealbumin Test System or a list of other products available for Wako, please contact Wako Diagnostics at toll free number: 877-714-1924, or by Email: Diagnostics@wakousa.com.
L-Type HDL-C Wako announces the availability of its L-Type HDL-C Assay test kit for the quantitative determination of high density lipoprotein cholesterol (HDL-C) in serum. The Wako L-Type HDL-C assay is based on an immunoinhibition. Antihuman ß-lipoprotein antibody in Reagent 1 binds to lipoproteins (LDL,VLDL, chylomicrons) other than HDL. The antigen antibody complexes formed, block enzyme reactions when Reagent 2 is added. Cholesterol esterase (CHE) and cholesterol oxidase (CO) in Reagent 2 react only with HDL-C. Hydrogen peroxide produced by the enzyme reacts with HDL-C to yield a blue color complex upon oxidase condensation with PDAOS and 4-aminoantipyrine in the presence of peroxidase (POD). By measurement of the blue color complex produced, the HDL-C concentration of the sample can be quantitated. This assay shows good correlation with the reference methods, no interference from coexisting substances, and is convenient with ready-to-use reagents. Stability and a high degree of linearity add to the benefits of this assay. This assay can be automated on your existing clinical chemistry instrumentation. Instrument applications are available
from Wako L-Type LDL-C Wako announces the availability of its L-Type LDL-C Assay test kit for the quantitative determination of low density lipoprotein cholesterol (LDL-C) in serum or plasma. The Wako L-Type LDL-C assay is homogeneous, with ready-to-use reagents which eliminates the preparatory steps or calculation and can be applied to automated chemistry analyzers. The Wako L-Type LDL-C assay follows a two step reaction which in the first step eliminates non-LDL cholesterol (Reagent 1) and in the second step a color reaction of the LDL cholesterol (Reagent 2). By measuring the blue color complex produced at 600 nm, the LDL-C of the sample is quantitated. Wako L-Type LDL-C assay has excellent performance in terms of linearity, precision, sensitivity, specificity and
correlation to the CDC reference method (Beta-quantification). Lipoprotein (a) Test Kit Wako announces the availability of its Autokit Lp(a) test kit for the quantitative determination of lipoprotein(a) in serum and plasma to aid with the risk assessment of coronary disease (CAD). The Wako Lp(a) assay utilizes turbidimetric immunoassay methodology in a simple one step, direct determination, with high specificity, no reactivity to Apo B or Plasminogen, excellent stability, and is applicable to your existing clinical chemistry analyzer. In the Wako TIA method, when a sample is mixed with the Buffer and the Antibody, Lp(a) in the sample combines specifically with anti-human lipoprotein(a) antibodies in the reagent to yield an insoluable aggregate that causes increased turbidity. The degree of turbidity can be measured optically and is proportional to the amount of Lp(a) protein mass in the sample. The Wako Lp(a) assay has excellent performance. The within-run precision has less than 3%CV. The linear range of the assay is 1-100mg/dL. Studies have been conducted that provide the expected value cutoff values for both Caucasians and African American populations. This will provide convenience to your laboratory in determining the expected values for your patient population. Instrument applications are available for most all clinical chemistry analyzer. CRP-HS Test Kit L-Type UIBC for use on most chemistry analyzers. Wako announces the release of its L-Type UIBC reagent kit for the quantitative determination of unsaturated iron binding capacity. The L-Type UIBC assay is based on a method that utilizes bathophenanthroline (BPT) as a chromogen. When a sample is mixed with Buffer containing a known excess amount of iron, unsaturated transferrin in serum quantitatively associates with iron in the Buffer and is converted to a saturated state. The portion of iron which remains unassociated is assayed through color development with ascorbic acid and bathophenanthroline disulfonic acid disodium salt. The unsaturated transferrin concentration in the sample can be determined by calculating the decreased amount of iron in the Buffer. UIBC concentrations are measured spectrophotometrically at 545nm. The Wako L-Type UIBC reagent is liquid, ready-to-use with superior stability. The assay is highly specific, has no significant interference by ascorbic acid, bilirubin, hemoglobin or intrafat, and has excellent precision. The Within-run precision is 0.65%CV and 0.85%CV at mean concentrations of 183.6mg/dL and 102.3mg/dL, respectively. The Total precision is 5.28%CV and 2.95%CV at mean concentrations of 115.8mg/dL and 187.6mg/dL, respectively. The minimum detectable limit is 2mg/dL with a linearity limit of 600mg/dL. This assay can be automated on your existing clinical chemistry instrumentation. Instrument applications are available
from Wako. Free Cholesterol Assay Kit for use on most automated chemistry Analyzers. Wako announces the availability of its Free Cholesterol C test kit for the quantitative determination of free cholesterol in serum. The Wako Free Cholesterol C assay utilizes an in vitro enzymatic colorimetric methodology, which eliminates the need for selective coprecipitaion and isolation procedures and can be automated using your existing clinical chemistry instrumentation. When used in conjunction with the Wako Total Cholesterol assay, the amount of cholesterol ester and the ester ratio (%) can be calculated. The Wako enzymatic method relies upon a simple procedure where free cholesterol in the test sample is allowed to react with cholesterol oxidase to generate hydrogen peroxide, which then reacts with phenol and 4-aminoantipyrine in the presence of peroxidase to produce a colored compound. The amount of free cholesterol is determined spectrophotometrically at 505nm. The Wako Free Cholesterol C assay has excellent performance. The within-run precision is 0.65%CV, 1.26%CV and 1.39%CV for mean concentrations of 105mg/dL, 84mg/dL and 60mg/dL, 
NEFA C Assay KitWako announces the availability of its NEFA C test kit for the quantitative determination of non-esterified (or free) fatty acids in serum or plasma (non-heparinized). The Wako NEFA C assay utilizes an in vitro enzymatic colorimetric methodology, which eliminates the need for an extraction procedure and can be automated using your existing clinical chemistry instrumentation. The Wako enzymatic method relies upon the acylation of coenzyme A(CoA) by the fatty acids in the presence of added acyl-CoA synthetase (ACS). The acyl-CoA produced is oxidized by added acyl-CoA oxidase (ACOD) with the generation of hydrogen peroxide. Hydrogen peroxide, in the presence of peroxidase (POD) permits the oxidative condensation of 3-methyl-N-ethyl-N-(b-hydroxyethyl)-aniline (MEHA) with 4-aminoantipyrine to form a purple color which can be measured spectrophotometrically at 550nm. The Wako NEFA C assay has excellent performance. The within-run precision is 2.7%CV, 1.1%CV and 1.1%CV for mean
concentrations of 0.33mmol/L, 0.62mmol/L and 0.99mmol/L, respectively. The linear range of the assay is 0-2.0 mmol/L. Phospholipids Assay Kit for use on automated chemistry analyzers Wako announces the availability of its Phospholipids B test kit for the quantitative determination of phospholipid content of serum and plasma. The Wako Phospholipid assay utilizes an in vitro enzymatic colorimetric methodology in a simple one step, direct determination, with high specificity, and readily automatable. In the Wako enzymatic method, phospholipids in serum or plasma (lecithin, lysolecithin, and sphingomyelin) are hydrolyzed to free choline by phospholipase D. In the presence of choline oxidase and peroxidase, the free choline is then estimated colorimetrically, by taking advantage of the color reaction with phenol and 4-aminoantipyrine at 505 nm. The Wako Phospholipids B assay has excellent performance. The within-run precision is 1.3%CV, 1.4%CV and 1.4 %CV
for mean concentrations of 132mg/dL, 228mg/dL and 324mg/dL, respectively. The linear range of the assay is 0-1000mg/dL.
There is no significant interference from bilirubin, hemoglobin, glucose, ascorbic acid, uric acid or anticoagulants
such as sodium citrate, heparin, ammonium oxalate and EDTA. .jpg) Total
Bilirubin AssayWako announces the availability of its Total Bilirubin V Assay test kit for the quantitative determination of total bilirubin concentration in serum. The Wako Total Bilirubin V assay is based on a chemical oxidation method, utilizing vanadate as the oxidizing agent. This assay shows good correlation with conventional methods, no interference from coexisting substances, is convenient ready-to-use reagents, stable and high linearity limit. In the Wako Total Bilirubin V assay, a sample is mixed with the reagent containing the detergent and the vanadate, at approximately pH 3, the total bilirubin in the sample is oxidized to biliverdin. This causes the absorbance of yellow, specific to bilirubin, to decrease. Therefore, the total bilirubin concentration in the sample can be obtained by measuring the absorbances before and after the vanadate oxidation. The Wako Total Bilirubin V assay has excellent performance. The Total precision studies give a 1.83%CV and 0.49%CV at mean concentrations of 0.60 mg/dL and 23.9 mg/dL, respectively. The linearity of the assay is up to 40 mg/dL. The assay has excellent correlation to alkaline azobilirubin methods. This assay can be automated on your existing clinical chemistry instrumentation. Instrument applications are available
from Wako. .jpg) D-
Bilirubin Assay not Effected by HemolysisWako announces the availability of its Direct Bilirubin V Assay test kit for the quantitative determination of direct bilirubin concentration in serum. The Wako Direct Bilirubin V assay is based on a chemical oxidation method, utilizing vanadate as the oxidizing agent. This assay shows good correlation with conventional methods, no interference from coexisting substances, is convenient ready-to-use reagents, stable and high linearity limit. In the Wako Direct Bilirubin V assay, a sample is mixed with the reagent containing the detergent and the vanadate, at approximately pH 3, the direct bilirubin in the sample is oxidized to biliverdin. This causes the absorbance of yellow, specific to bilirubin, to decrease. Therefore, the direct bilirubin concentration in the sample can be obtained by measuring the absorbances before and after the vanadate oxidation. The Wako Direct Bilirubin V assay has excellent performance. The total precision studies give a 3.95%CV and 1.05%CV at mean concentrations of 0.19mg/dL and 16.3mg/dL, respectively. The linearity of the assay is up to 20 mg/dL. The assay has excellent correlation to alkaline azobilirubin methods. Using the Wako Bilirubin Calibrator, which has an assigned value for Direct Bilirubin, it is not necessary to calibrate factored from the Total Bilirubin assay and can be done directly. This assay can be automated on your existing clinical chemistry instrumentation. Instrument applications are available from Wako. |
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